The elimination of 3:4-benzpyrene from the animal body after subcutaneous injection

Publisher Website
Google Scholar
Abstract
The fluorescent metabolite of benzpyrene excreted in the feces of the rat was purified by chromato-graphic adsorption on alumina and high vacuum sublimation. In this way, a small amt. of crystalline material was obtained. The M.P. 190-196[degree] decomp. was detd. by a modified micro M.P. technique, in which the crystals were heated on a Wood''s metal bath. The solubility of the compound indicated that it was phenolic, and an X ray crystallographic examination showed that the mol. wt. of the compound is of the right order of magnitude for a mono-hydroxy derivative of benzpyrene, assuming the compound to be phenolic. Consideration of the arrangement of the molecules in the crystal indicated the most likely positions for the hydroxy group in the molecule. Crystallographic examination showed that the metabolite of benzpyrene is not identical with 6-hydroxy or 4''-hydroxy-3:4-benzpyrene.