Transcriptional organization of bovine papillomavirus type 1

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Abstract
Multiple bovine papillomavirus type 1 (BPV-1)-specific polyadenylated RNA species in a BPV-1-infected bovine fibropapilloma were identified and mapped. All of the RNA species were transcribed from the same DNA strand of the BPV-1 genome. Five RNA species previously identified in BPV-1-transformed mouse cells were also present in the bovine fibropapilloma. These 5 spp. measured 1050, 1150, 1700, 3800 and 4050 bases, mapped within the 69% transforming segment of the BPV-1 genome, and shared a 3'' coterminus at 0.53 map units (m.u.). The 5'' ends of the bodies of these distinct transcripts were located a .apprx. 0.03, 0.09, 0.34, 0.39 and 0.41 m.u. Additional polyadenylated RNA species not present in BPV-1-transformed mouse cells were specific for the BPV-1-infected bovine fibropapilloma and measured 1700, 3700, 3800, 6700 and 8000 bases. These wart-specific species shared a 3'' coterminus at 0.90 m.u. The 5'' termini of the bodies of the 1700- and 3800-base species mapped at 0.71 and 0.42 m.u., respectively. Exonuclease VII analysis failed to reveal any internal splicing in these 2 species: the presence of small remote 5'' leader sequences could not be ruled out. The 3700-base species hybridized to DNA fragments from the 69% transforming segment as well as from the 31% nontransforming segment of the BPV-1 genome; this species was not precisely mapped. The 5'' termini of the 2 largest RNA species (6700 and 8000 bases in size) were located at .apprx. 0.01 and 0.90 m.u., respectively. Since the 5'' ends of these mapped adjacent to a TATAAA sequence which could possibly serve as an element of a transcriptional promoter, it is possible that one or both of these species represent nonspliced precursor RNA molecules.