Wide‐field optically sectioning fluorescence microscopy with laser illumination
- 1 January 2000
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 197 (1) , 1-4
- https://doi.org/10.1046/j.1365-2818.2000.00656.x
Abstract
We describe an extremely simple method by which optically sectioned fluorescence images may be obtained with conventional microscopes using laser illumination. A one‐dimensional grid pattern is introduced into the illumination system, together with a rotating ground glass diffuser. This causes an image of the grid pattern to be projected into the specimen. Images taken at three spatial positions of the grid are processed in a simple manner to provide optically sectioned images of fluorescent specimens.Keywords
This publication has 8 references indexed in Scilit:
- Imaging Protein Kinase Cα Activation in CellsScience, 1999
- Real‐time three‐dimensional imaging of macroscopic structuresJournal of Microscopy, 1998
- Method of obtaining optical sectioning by using structured light in a conventional microscopeOptics Letters, 1997
- Rapid acquisition, analysis, and display of fluorescence lifetime-resolved images for real-time applicationsReview of Scientific Instruments, 1997
- Confocal fluorescence microscopy using spectral and lifetime information to simultaneously record four fluorophores with high channel separationJournal of Microscopy, 1997
- Photon correlation system for fluorescence lifetime measurementsReview of Scientific Instruments, 1995
- Handbook of Biological Confocal MicroscopyPublished by Springer Nature ,1995
- Lifetime-selective fluorescence imaging using an rf phase-sensitive cameraReview of Scientific Instruments, 1991