Use of the Hydroxamic Acid Reaction for Determining Pectinesterase Activity
- 1 January 1958
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 33 (3) , 129-131
- https://doi.org/10.3109/10520295809111836
Abstract
The technique is: React pectinesterase contained on filter paper discs on a pectin-agar plate, flood the plate with a solution of hydroxylamine hydrochloride and then a solution of sodium hydroxide, acidify with hydrochloric acid, and then add a ferric chloride solution. The areas of substrate, desterified by the enzyme, appear as clear zones on a red background. The formation of the insoluble, red-colored ferric-pectin hydroxamic acid is characteristic of the presence of pectin carbomethoxy (methyl ester). Areas which are not colored are indicative of pectinesterase activity and this test can be used for qualitative and semi-quantitative test for approximately 1 × 10-3 to 1 × 10-4 pectinesterase units.Keywords
This publication has 2 references indexed in Scilit:
- Plant Tissue Analysis, Test for Pectin Based on Reaction of Hydroxamic Acids with Ferric IonJournal of Agricultural and Food Chemistry, 1955
- The enzymic degradation of pectin and other polysaccharides. II—Application of the ‘Cup-plate’ assay to the estimation of enzymesJournal of the Science of Food and Agriculture, 1953