A CORRELATION BETWEEN TUMOR-CELL ATTACHMENT TO A SUBSTRATE AND PROLIFERATIVE CHARACTERISTICS

  • 1 January 1984
    • journal article
    • research article
    • Vol. 6  (1) , 27-32
Abstract
Cloned cell lines (17) of transformed mouse fibroblasts were used for the evaluation of a correlation between 3 traits of malignancy; cloning efficiency in semisolid medium (CE); cell dose inducing tumors in 50% of inoculated animals (TD50); degree of cell attachment to a substrate (RT50.sbd.rounding time for 50% of the cells under moderate cooling, 18.degree. C). An increase in RT50 (better attachment of the cells to the substrate) is accompanied by a decrease in CE and an increase in TD50 (correlation coefficients p = -0.76 and p = 0.58, respectively). A negative correlation between CE and TD50 was also revealed (.rho. = -0.68). An increase in the percentage of cells containing actin filament bundles was found in cell clones better attached to the substrate. Mechanisms of disturbances in proliferation and attachment of malignant cells are discussed.