Hypogin, a novel antifungal peptide from peanuts with sequence similarity to peanut allergen
- 1 April 2001
- journal article
- research article
- Published by Wiley in Chemical Biology & Drug Design
- Vol. 57 (4) , 330-336
- https://doi.org/10.1046/j.1397-002x.2001.00874.x
Abstract
A protein designated hypogin, with a prominent suppressive action on the growth of the fungi Mycosphaerella arachidicola, Fusarium oxysporum and Coprinus comatus, was isolated from seeds of the peanut Arachis hypogaea. The protein inhibited human immunodeficiency virus (HIV) reverse transcriptase and enzymes associated with HIV infection including α‐glucosidase and β‐glucosidase. The proliferative response of mouse splenocytes was attenuated in the presence of the protein. The protein exhibited a molecular mass of 7.2 kDa in tricine gel electrophoresis and gel filtration on Superdex 75 and an N‐terminal sequence resembling peanut allergen Ara H1. The isolation procedure involved affinity chromatography on Affi‐gel blue gel and ion‐exchange chromatography on CM−Sepharose. The protein was adsorbed in both chromatographic media.Keywords
This publication has 18 references indexed in Scilit:
- SativinLife Sciences, 2000
- Dolichin, a New Chitinase-like Antifungal Protein Isolated from Field Beans (Dolichos lablab)Biochemical and Biophysical Research Communications, 2000
- First Chromatographic Isolation of an Antifungal Thaumatin-like Protein from French Bean Legumes and Demonstration of Its Antifungal ActivityBiochemical and Biophysical Research Communications, 1999
- Purification and Characterization of Novel Ribosome Inactivating Proteins, Alpha- and Beta-Pisavins, from Seeds of the Garden PeaPisum SativumBiochemical and Biophysical Research Communications, 1998
- A comparison of human immunodeficiency virus type 1 inhibition by partially purified aqueous extracts of chinese medicinal herbsLife Sciences, 1997
- A highly efficient procedure for purifying the ribosome-inactivating proteins α- and β-momorcharins from seeds. n-terminal sequence comparison and establishment of their n-glycosidase activityLife Sciences, 1996
- Isolation and characterization of two distinct lectins with antiproliferative activity from the cultured mycelium of the edible mushroom Tricholoma mongolicumInternational Journal of Peptide and Protein Research, 1995
- Immunomodulatory and antitumor activities of a polysaccharide-peptide complex from a mycelial culture of Tricholoma sp., a local edible mushroomLife Sciences, 1995
- Ribosome-inactivating proteins from plantsBiochimica et Biophysica Acta (BBA) - Reviews on Biomembranes, 1993
- An Efficient mRNA‐Dependent Translation System from Reticulocyte LysatesEuropean Journal of Biochemistry, 1976