Splanchnic Nerve Transection Abolishes the Age‐Dependent Increase of Neuropeptide Y‐Like Immunoreactivity in Rat Adrenal Gland

Abstract
Using an antiserum directed against porcine neuropeptide Y (NPY), a high concentration of NPY immunoreactivity (NPY-IR) was detected in rat adrenal gland. The level of NPY-IR in the adrenal gland was found to increase considerably with age. Biochemical characterization by reverse-phase HPLC indicated that this increase was due to accumulations of NPY and another molecular form of NPY-like immunoreactive peptide. Chronic denervation of the splanchnic nerve abolished this age-dependent increase of NPY-IR in rat adrenal gland.