Effects of Ionizing Radiation on the Fine Structure and Function of the Intestinal Epithelium of the Mouse: I. Villus Epithelium

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Abstract
Mice were x-irradiated with 200, 800 and 3000 rads which produce, respectively, slight, severe but reversible, and irreversible damage to the intestinal mucosa. Animals were sacrificed 1/2 hour to 5 days after irradiation; corn oil was given by gavage 1 hour before sacrifice. The lower ileum was studied with light microscope and electron microscope for general pathology and for alterations of oil absorption. The cell membrane of mature cells shows evidence of softening within 10 minutes after irradiation with 200 rads. Mitochondria show early alterations, and more severe changes in cells which have matured after having been irradiated while in proliferative state. Nuclear pathology and depletion of ribonucleoprotein granules are pronounced in cells of this latter type. Corn oil absorption is about normal as long as the villus is covered by cells which were mature at irradiation, while absorption is slightly decreased and transport considerably impaired when the villus is covered by cells which have matured after irradiation in proliferative state.