A New Method of Determining the Fraction of Hypoxic Cells in a Transplantable Murine Sarcoma

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Abstract
Combined nitrogen mustard and radiation treatments of the KHT [mouse] sarcoma in vivo indicated that a certain dose of nitrogen mustard can kill all oxygenated cells in the tumor while killing few, if any, hypoxic cells. This finding allowed nitrogen mustard treatment to be used as a way of selectively removing oxygenated cells in the tumor so that the radiation response of hypoxic cells can be studied directly. Using this nitrogen mustard technique the hypoxic fraction was 0.30 as compared to a value of 0.13 obtained using the more conventional technique of comparing anoxic and air breathing survival data following the same dose of radiation. Chronically hypoxic cells had a smaller shoulder on their radiation survival curve than acutely hypoxic cells.

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