Determination of N 1-Methylnicotinamide in Urine by High-Pressure Liquid Chromatography
- 1 June 1977
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of Chromatographic Science
- Vol. 15 (6) , 215-217
- https://doi.org/10.1093/chromsci/15.6.215
Abstract
This paper reports a precise method that is shorter than previously reported methods for the quantitative determination of N1-methylnicotinamide (MNA) in urine. The method employs a single column chromatographic isolation step, followed by high-pressure liquid chromatographic (HPLC) analysis. Potential interfering substances present in urine are removed during the column chromatography step. The combined MNA fractions eluted from this column were collected and concentrated for quantitative assay of MNA by HPLC. HPLC analysis was effected in less than 15 min using a strong cation- exchange column eluted with 0.25 M ammonium dihydrogen phosphate (pH 4.3). Linearity of MNA detection by HPLC. at 254 nm extended below 20 ng, with an average recovery of 101% for 150, 250 and 500 μg MNA added to 5 ml of urine.This publication has 5 references indexed in Scilit:
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