2 rearranges with all functional Jλ segments in the mouse

Abstract
We have analyzed 210 λ‐producing hybridomas derived from lipopolysaccharidestimulated spleen cells from a single x‐suppressed mouse. All were classified as λ1, λ2 or λ3 with the exception of four unusual lines. Two of these were due to Vλ21 and the other two to Vλ23 rearrangements. The lines were clonally independent since the point of VJ recombination in each one was different. Southern blot analysis of the Vλ21‐producing lines showed no evidence for an inversion. Under the assumption of a simple deletion model of rearrangement these findings place the Vλ2 cluster upstream of the Vλ1 cluster oriented in the same direction.