Mouse monoclonal antibodies against the idiotype of human B cell non‐Hodgkin lymphomas: production, characterization and use to monitor the progress of disease

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Abstract
Four monoclonal anti‐idiotype (Id) antibodies against B cell non‐Hodgkin lymphomas have been made by a simple and rapid procedure for hybridoma production that does not use isolated Id‐carrying immunoglobulin. Mice were immunized with plasma membranes from the tumor cells. Screening of hybridoma colonies using an autologous T cell line and a cell line bearing the same immunoglobulins as the malignant lymphocytes as well as the capping phenomenon on the patient's own tumor cells, allowed selection of hybridomas producing anti‐Id antibodies. These anti‐Id antibodies were used to demonstrate variations in the circulating malignant cell population and fluctuations in the level of circulating free Id.