DETERMINATION OF PLASMA CYCLIC-AMP WITH AUTOMATED RADIOIMMUNOASSAY SYSTEM (GAMMA-FLO)

  • 1 January 1982
    • journal article
    • research article
    • Vol. 8  (5) , 297-307
Abstract
The Gamma-flo automated radioimmunoassay system was adapted to analyze for cAMP in plasma. Direct analysis of plasma samples was not possible due to an interfering substance(s) in plasma. Evidence suggested that the interfering substance is human serum albumin. An efficient, relatively simple method was developed by applying the acetylation procedure to ultrafiltrates of plasma samples and utilizing the Gamma-flo system for rapid radioimmunoassay (50 samples/h) of cAMP content in the filtrates. Coefficient of variation within assays averaged 3% and interassay coefficient of variation was 4.7%. Recovery of cAMP from plasma into the ultrafiltrate ranged from 93-100%. Increments of cAMP added to plasma were recovered linearly up to .apprx. 60 pmol/ml, the highest concentration tested.