Phylogenetic Relationships Between Mycoplasmas and Other Procaryotes Based upon the Electrophoretic Behavior of Their Ribosomal Ribonucleic Acids
- 1 July 1977
- journal article
- research article
- Published by Microbiology Society in International Journal of Systematic and Evolutionary Microbiology
- Vol. 27 (3) , 185-193
- https://doi.org/10.1099/00207713-27-3-185
Abstract
The migration of ribosomal ribonucleic acids (rRNA's) from bacteria, bacterial L forms, mycoplasmas, and acholeplasmas in nondenaturing and formamide gels has been examined. Mycoplasmal and acholeplasmal RNAs migrate in a pattern distinct from each other and from bacteria in nondenaturing gels. The differences observed appear to be mainly due to conformational differences in the molecules. When examined under denaturing conditions in formamide gels, the 23S RNAs of all organisms examined comigrated. The 16S RNAs of mycoplasmas and acholeplasmas comigrated and appear to be of a smaller molecular weight than bacterial 16S RNA. It is suggested that the differences observed are indicative of significant phylogenetic divergence between mycoplasmas and bacteria or bacterial L forms.This publication has 3 references indexed in Scilit:
- Molecular weight estimation and separation of ribonucleic acid by electrophoresis in agarose-acrylamide composite gelsBiochemistry, 1968
- Biosynthetic precursors of 30 S and 50 S ribosomal particles in Escherichia coliBiochemistry, 1968
- Sedimentation coefficients of RNA from 70S and 80S ribosomes.Proceedings of the National Academy of Sciences, 1967