Endogenous and Silencing-Associated Small RNAs in Plants[W]
Top Cited Papers
- 1 July 2002
- journal article
- Published by Oxford University Press (OUP) in Plant Cell
- Vol. 14 (7) , 1605-1619
- https://doi.org/10.1105/tpc.003210
Abstract
A large set of endogenous small RNAs of predominantly 21 to 24 nucleotides was identified in Arabidopsis. These small RNAs resembled micro-RNAs from animals and were similar in size to small interfering RNAs that accumulated during RNA silencing triggered by multiple types of inducers. Among the 125 sequences identified, the vast majority (90%) arose from intergenic regions, although small RNAs corresponding to predicted protein-coding genes, transposon-like sequences, and a structural RNA gene also were identified. Evidence consistent with the derivation of small RNAs of both polarities, and from highly base-paired precursors, was obtained through the identification and analysis of clusters of small RNA loci. The accumulation of specific small RNAs was regulated developmentally. We propose that Arabidopsis small RNAs participate in a wide range of post-transcriptional and epigenetic events.Keywords
This publication has 71 references indexed in Scilit:
- Signaling in plants by intercellular RNA and protein movementGenes & Development, 2002
- Micro-RNAsThe Journal of cell biology, 2002
- Analysis of the 5S RNA Pool in Arabidopsis thaliana: RNAs Are Heterogeneous and Only Two of the Genomic 5S Loci Produce Mature 5S RNAGenome Research, 2001
- On the Role of RNA Amplification in dsRNA-Triggered Gene SilencingCell, 2001
- Glimpses of a Tiny RNA WorldScience, 2001
- An Abundant Class of Tiny RNAs with Probable Regulatory Roles in Caenorhabditis elegansScience, 2001
- A Role for the RNase III Enzyme DCR-1 in RNA Interference and Germ Line Development in Caenorhabditis elegansScience, 2001
- Expanded sequence dependence of thermodynamic parameters improves prediction of RNA secondary structureJournal of Molecular Biology, 1999
- The Cold Shock Domain Protein LIN-28 Controls Developmental Timing in C. elegans and Is Regulated by the lin-4 RNACell, 1997
- CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucleic Acids Research, 1994