AMOUNTS AND TURNOVER RATES OF BRAIN PROTEINS IN MORPHINE‐TOLERANT MICE

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Abstract
Abstract— Mice were injected intracerebrally with radioactive leucine 30 min before decapitation. A double‐label technique was used; e.g. [3H]leucine in control, untreated mice and [14C]leucine in morphine‐tolerant (dependent) mice. Proteins were extracted sequentially from mouse brain with aqueous buffer, Triton X‐100, and sodium lauryl sulphate. Each extract was subjected to electrophoresis on discontinuous, porosity‐gradient acrylamide gels. When the protein patterns from untreated, acutely morphinized mice were compared with those from morphine‐tolerant (dependent) mice, we observed no differences in the amounts of protein or of radioactivity in any of the 55 discrete bands.