Ganglioside GD1b is the target antigen for a biclonal IgM in a case of sensory‐motor axonal polyneuropathy: Involvement of N‐acetylneuraminic acid in the epitope
- 1 July 1992
- journal article
- case report
- Published by Wiley in Annals of Neurology
- Vol. 32 (1) , 18-23
- https://doi.org/10.1002/ana.410320105
Abstract
We report on a 54‐year‐old man with a sensory‐motor polyneuropathy associated with a biclonal IgM‐k gammopathy, which reacted with the ganglioside GD1b. Examination of nerve biopsy specimens showed some reduction in the density of myelinated fibers and axonal degeneration with a loss of large fibers and a relative increase in the density of small fibers. Immunodetection on thin‐layer chromatography of the glycolipid antigens showed strong reactivity of the patient's serum IgM‐k with GD1b ganglioside and weak binding to GD1a. The biclonal IgM antibodies did not react with GM1, asialo‐GM1, GT1b, GD2, or GD3. Indirect immunofluorescence staining showed binding of IgM‐k mainly in a crescent‐like pattern on the internal side of myelin sheaths, which could correspond either to an enlarged periaxonal (adaxonal) space or to the internal mesaxon or to both. The immunostaining was abolished after absorption of the serum with GD1b.Keywords
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