Über experimentelle Alkaptonurie bei der weißen Ratte

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Abstract
Exptl. alcaptonuria was produced in white rats by feeding them daily 0.4 g. l-tyrosine or 0.9 g. l-phenylalanine/ 100 g. of body wt. The amino acids were mixed with the diet of roast veal. The time required to produce alcaptonuria was usually about 1 wk., but varied with the different animals and with the amts. of amino acid given. The maximum excretion of homogentisic acid occurred 6-8 hrs. after the acids were fed. Feeding of a single large dose of these acids did not cause alcaptonuria. Neither was alcaptonuria caused by feeding d-phenylalanine nor by d,l-tyrosine unless the d,l-tyrosine was fed in very large doses. To isolate the homogentisic acid, 280 cc. of the rat urine, which gave a strongly positive test for the acid, were acidified with HC1, a little sulfurous acid added, and the mixture extracted with peroxide free ether. The ether was evaporated, the residue dissolved in H2O, and the Pb salt (m. 214[degree]) prepared, which was decomposed with H2S, and homogentisic acid (m. 146[degree]) obtained.

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