CHEMICALLY DEFINED MEDIA FOR SYNNEMATIN PRODUCTION

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Abstract
Optimum conditions for growth and production of synnematin on chemically defined media by Cephalosporium salmosynnematum and Emericellopsis terricola var. glabra were investigated. A pH of about 6 was optimum for growth of C. salmosynnematum and a pH range 4.8 to 6.5 was optimum for the growth of E. terricola var. glabra. Good growth was obtained on media containing glucose, sucrose, or maltose and inorganic or organic nitrogen sources. Biotin was necessary for the growth of E. terricola var. glabra. The maximum rate of synnematin production was obtained under conditions which supported a very slow growth rate. A medium containing glucose, ammonium sulfate, calcium carbonate, salts, (and biotin in the case of E. terricola var. glabra) was devised which supported a rapid growth rate during the growth phase and a slow growth rate during the synnematin production phase. The slow growth rate during the synnematin producing phase was obtained by feeding glucose intermittently to the fermentation after the end of the growth phase. Synnematin yields obtained with C. salmosynnematum (36 units/ml) were lower than those obtained with E. terricola var. glabra (300 units/ml). No improvement in synnematin yields was obtained when DL-[alpha]-amino-adipic acid, other amino acids, or vitamins were added to the medium.