c‐yes and bc1‐2 genes located on 18q21.3 in a follicular lymphoma cell line carrying a t(14;18) chromosomal translocation

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Abstract
The c‐yes‐1 and bc1‐2 genes have been independently mapped at the same position of the chromosome band, 18q21.3, which is a breakpoint in the t(14; 18) chromosomal translocation. High‐molecular‐weight genomic DNAs and mRNA isolated from a follicular lymphoma cell line FL‐18 and an Epstein‐Barr virus harboring FL‐18EB cell line, which carried the t(14;18), were analyzed. Although there was no detectable c‐yes rearrangement, the chromosome 18‐specific probe b (bc1‐2) revealed one rearranged fragment after EcoRI, BamHI, PstI and SstI digestion. Therefore, breakage of chromosome 18 in the t(14;18) occurred at the SstI fragment containing the probe b sequence on the bc1‐2 gene. The transcript of c‐yes was undetectable, whereas active transcription of bc1‐2 was observed in the FL‐18 and FL‐18EB cell lines at a higher level than in other types of lymphoma cell line. These data suggest that the c‐yes‐1 is not involved in the t(14;18), and the 2 genes located at 18q21.3 are independent sequences.