Action of esterase in the presence of organic solvents

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Abstract
A method for determining the activity of esterase preparations is described in which the given preparations of solutions of the substrates in organic solvents are added to the esterase preparations. The degree of esterification is determined in the solvent phase by the drop in acidity and alcohol concn. The velocity of reaction, v (number of millimols of ester per liter formed in the non-aqueous phase during the first hour of reaction), rises with increasing concn. of acid; when the concn. of alcohol is increased the reaction velocity rises to a maximum at molar concn. of alcohol, thereafter falling. This influence of concns, of butyric acid and BuOH on v is explained to some extent by the aid of coefficients of partition of the substrates between (e.g.) the benzene and the aqueous phase.

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