Association of the v-crk oncogene product with phosphotyrosine-containing proteins and protein kinase activity.
- 1 April 1990
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 87 (7) , 2638-2642
- https://doi.org/10.1073/pnas.87.7.2638
Abstract
An antiserum specific for P47gag-crk, the oncogene product of avian sarcoma virus CT10, was used to investigate possible crk-binding proteins. In in vitro kinase assays, four proteins were phosphorylated in anti-crk immunoprecipitates. Tyrosine, serine, and threonine residues were phosphorylated. A number of tyrosine-phosphorylated proteins were identified in anti-crk immunoprecipitates of 32P-labeled cells, including the three major phosphotyrosine-containing proteins of CT10-infected cells. These three proteins also bound to bacterially synthesized crk protein. These results suggest that the crk transforming proteine can stably associate with both endogenous kinases and cellular kinase substrates.This publication has 42 references indexed in Scilit:
- A Second Type of Rat Phosphoinositide-specific Phospholipase C Containing a src-related Sequence Not Essential for Phosphoinositide-hydrolyzing ActivityJournal of Biological Chemistry, 1989
- PDGF induction of tyrosine phosphorylation of GTPase activating proteinNature, 1989
- Platelet-derived growth factor (PDGF) binding promotes physical association of PDGF receptor with phospholipase C.Proceedings of the National Academy of Sciences, 1989
- Stable association of activated pp60src with two tyrosine-phosphorylated cellular proteins.Molecular and Cellular Biology, 1989
- Direct activation of the serine/threonine kinase activity of raf-1 through tyrosine phosphorylation by the PDGF β-receptorCell, 1989
- Platelet-derived growth factor induces rapid and sustained tyrosine phosphorylation of phospholipase C-gamma in quiescent BALB/c 3T3 cells.Molecular and Cellular Biology, 1989
- Phospholipase C-γ is a substrate for the PDGF and EGF receptor protein-tyrosine kinases in vivo and in vitroCell, 1989
- EGF induces tyrosine phosphorylation of phospholipase C-II: A potential mechanism for EGF receptor signalingCell, 1989
- Detection of phosphotyrosine-containing proteins in polyomavirus middle tumor antigen-transformed cells after treatment with a phosphotyrosine phosphatase inhibitor.Molecular and Cellular Biology, 1987
- Isolation of antibodies for phosphotyrosine by immunization with a v-abl oncogene-encoded protein.Molecular and Cellular Biology, 1985