Adeno‐associated virus‐mediated gene transfer into human retinal pigment epithelium cells
- 1 May 1998
- journal article
- Published by Wiley in Australian and New Zealand Journal of Ophthalmology
- Vol. 26 (S1) , S77-S79
- https://doi.org/10.1111/j.1442-9071.1998.tb01381.x
Abstract
Purpose: Adeno‐associated virus (AAV) is emerging as a promising vector for gene therapy. Method: To determine the ability of recombinant AAV (rAAV) to express and integrate exogenous DNA into human retinal pigment epithelium (RPE) cells, a rAAV‐GFP vector containing the green fluorescent protein (gfp) and neomycin resistance (neor) genes was constructed and used to transduce RPE 407A cell line. Results: Fluorescent RPE cell clones were obtained and were confirmed to still be expressing GFP after 24 passages (3.5 months). Conclusion: Adeno‐associated virus‐based vectors are able to efficiently transduce and stably persist in RPE cells.Keywords
This publication has 12 references indexed in Scilit:
- A novel 165-base-pair terminal repeat sequence is the sole cis requirement for the adeno-associated virus life cycleJournal of Virology, 1997
- Efficient long-term gene transfer into muscle tissue of immunocompetent mice by adeno-associated virus vectorJournal of Virology, 1996
- Gene transfer into the mouse retina mediated by an adeno-associated viral vectorHuman Molecular Genetics, 1996
- Biology of Adeno-associated VirusPublished by Springer Nature ,1996
- AAV as a viral vector for human gene therapyMolecular Biotechnology, 1995
- Organization of adeno-associated virus DNA in latently infected detroit 6 cellsVirology, 1989
- Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5Journal of General Virology, 1977