c-Cbl is downstream of c-Src in a signalling pathway necessary for bone resorption

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Abstract
THE primary defect in mice lacking the c-src gene is osteopetrosis, a deficiency in bone resorption by osteoclasts1. Osteoclasts express high levels of the c-Src protein2,3 and the defect responsible for the osteopetrotic phenotype of the c-src-deficient (src−) mouse is cell-autonomous and occurs in mature osteoclasts4,5. However, the specific signalling pathways that require c-Src expression for normal osteoclast activity have not been elucidated. We report here that the proto-oncogene product c-Cbl is tyrosine-phosphorylated in a Src-dependent manner in osteoclasts, where the two proteins colocalize on some vesicular structures. In vitro bone resorption by osteoclast-like cells (OCLs) is inhibited by both c-src and c-cbl antisense oligonucleo-tides. Furthermore, tryosine phosphorylation of c-Cbl and the localization of c-Cbl-containing structures to the peripheral cytoskeleton are impaired in resorption-deficient c-src− OCLs, as well as in wild-type OCLs that have been treated with c-src antisense oligonucleotides. These results indicate that c-Cbl may act downstream of c-Src in a signalling pathway that is required for bone resorption.

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