Conjugated Hemoglobin: Rightshifted Dextran-Hemoglobin as Blood Substitute
- 1 January 1988
- journal article
- research article
- Published by Taylor & Francis in Biomaterials, Artificial Cells and Artificial Organs
- Vol. 16 (1-3) , 237-245
- https://doi.org/10.3109/10731198809132573
Abstract
Covalent modification of hemoglobin and dextran-hemoglobin by reductive alkylation under aerobic conditions with periodated inositol tetrakisphosphate brought about a rightshifting of their oxygen dissociation curves. The rightshifted dextran-hemoglobin conjugate provided adequate oxygen delivery in macaques exchange-transfused to 90% with respect to their erythrocyte content. The conjugate did not exhibit the nephrotoxicity of free hemoglobin. It was also more resistant to precipitation, and therefore more amenable to viral sterilisation, by ethanol.Keywords
This publication has 17 references indexed in Scilit:
- Synthesis of monomethoxypolyoxyethylene-bound haemoglobinsTetrahedron, 1984
- Hemoglobin-inulin conjugate as an oxygen carrying blood substituteBiochemical and Biophysical Research Communications, 1983
- Oxygen-binding and immunological properties of complexes between dextran and animal haemoglobinsBiochemical Journal, 1981
- Synthesis of polymer-bound hemoglobin samplesTetrahedron, 1981
- Modification of human hemoglobin with polyethylene glycol: A new candidate for blood substituteBiochemical and Biophysical Research Communications, 1980
- Polyvinylpyrrolidon-gebundenes Hämoglobin als ein Sauerstoff übertragender BlutersatzstoffJournal of Molecular Medicine, 1979
- Studies on Avian Erythrocyte MetabolismEuropean Journal of Biochemistry, 1977
- Synthesis of soluble dextran–hemoglobin complexes of different molecular sizesCanadian Journal of Biochemistry, 1977
- Soluble dextran-hemoglobin complex as a potential blood substitute.Proceedings of the National Academy of Sciences, 1976
- Pyridoxal Compounds as Specific Reagents for the α and β N-Termini of HemoglobinProceedings of the National Academy of Sciences, 1973