Molecular characterization, relatedness and antifungal susceptibility of the basidiomycetous hormographiella species and Coprinus cinereus from clinical and environmental sources
- 1 July 1996
- journal article
- research article
- Published by Springer Nature in Antonie van Leeuwenhoek
- Vol. 70 (1) , 49-57
- https://doi.org/10.1007/bf00393569
Abstract
Hormographiella-like strains, isolated from different natural substrates and producing sclerotia and occasionally basidiomata of Coprinus cinereus, were compared morphologically and using molecular techniques with clinical strains of Hormographiella aspergillata and H. verticillata. Analysis of restriction fragment length polymorphisms of ribosomal and mitochondrial-like DNA confirmed interspecific differences between H. aspergillata and H. verticillata, supporting the morphological data, and helped demonstrate that H. aspergillata is the anamorph of C. cinereus. The latter was confirmed also by crossing tests. The analysis of the mtDNA restriction profiles revealed intraspecific variability in C. cinereus, which allowed differentiation of clinical and environmental strains. Due to the implication of C. cinereus and Hormographiella in human opportunistic infections, the antifungal susceptibility test is included. Results show that all strains were susceptible to miconazole, itraconazole and ketoconazole but not to flucytosine and fluconazol. Susceptibility against amphotericin B was variable; while H. verticillata was susceptible, four out of seven C. cinereus strains tested were resistant.Keywords
This publication has 16 references indexed in Scilit:
- Molecular differentiation of Keratinomyces (Trichophyton) speciesAntonie van Leeuwenhoek, 1996
- Molecular differentiation of two races of Fusarium oxysporum special form cubenseLetters in Applied Microbiology, 1994
- Typing truffle species by PCR amplification of the ribosomal DNA spacersMycological Research, 1994
- Characterization of Some Cylindrocladium Species with Three-Septate Conidia Using Morphology, Isozyme Banding Patterns and DNA PolymorphismsSystematic and Applied Microbiology, 1993
- Restriction polymorphisms in the internal transcribed spacers and 5.8S rDNA ofSaccharomycesCurrent Microbiology, 1992
- Basidiomycetous (Mushroom) Infection of the Maxillary SinusOtolaryngology -- Head and Neck Surgery, 1990
- Maxillary sinus infection caused by the homobasidiomycetous fungus Schizophyllum communeJournal of Clinical Microbiology, 1986
- Endocarditis Caused By A Coprinus Species: A Fungus Of The Toadstool GroupJournal of Medical Microbiology, 1971
- CONIDIOPHORES, CONIDIA, AND CLASSIFICATIONCanadian Journal of Botany, 1953
- A Basidiomycete Probably Causing Onychomycosis*Journal of Investigative Dermatology, 1950