Direct visualization of colloidal gold‐bound molecules and a cell‐surface receptor by ultrahigh‐resolution scanning electron microscopy
- 1 March 1991
- journal article
- Published by Wiley in Journal of Microscopy
- Vol. 161 (3) , 455-461
- https://doi.org/10.1111/j.1365-2818.1991.tb03103.x
Abstract
The structure of protein A‐coated colloidal gold particles, and of macrophage cell‐surface receptors conjugated with immunogold particles, was studied using an ultrahigh‐resolution scanning electron microscope. Protein A, when conjugated with 15‐nm gold, formed a coat completely surrounding the particle. Particles conjugated with both protein A and immunoglobulin G (IgG) were similar, but with additional protrusions formed by the IgG. IgG molecules directly bound to gold were resolved sometimes as complexes of three units, sometimes as more filamentous, V‐shaped structures. On the cell surface of a macrophage reacted with a monoclonal antibody to Mac‐1 antigen (the murine C3bi receptor) followed by protein A‐gold, gold particles were seen to be linked via the IgG to the receptor, visualized as a round granule.Keywords
This publication has 11 references indexed in Scilit:
- Application of an ultrahigh‐resolution scanning electron microscope (UHS‐T1) to biological specimensJournal of Electron Microscopy Technique, 1989
- Scanning electron microscopy of cell surface antigens labeled with colloidal goldJournal of Anatomy, 1986
- A novel approach for scanning electron microscopy of colloidal gold-labeled cell surfaces.The Journal of cell biology, 1984
- Identification of the C3bi receptor of human monocytes and macrophages by using monoclonal antibodies.Proceedings of the National Academy of Sciences, 1983
- Cell growth and differentiation in vitro in mouse macrophages transformed by a tsA mutant of simian virus40. I. Cellular response in proliferative and phagocytic activities to the shift of temperature differs depending on the culture state in mouse bone marrow cells transformed by the tsA640 mutant of simian virus 40Journal of Cellular Physiology, 1983
- Anti-Mac-1 selectively inhibits the mouse and human type three complement receptor.The Journal of Experimental Medicine, 1982
- Cell surface distribution of fibronectin in cultures of fibroblasts and bladder derived epithelium: SEM‐immunogold localization compared to immunoperoxidase and immunofluorescenceJournal of Microscopy, 1981
- Colloidal gold granules as markers for cell surface receptors in the scanning electron microscopeCellular and Molecular Life Sciences, 1975
- Morphology of colloidal gold, ferritin and anti-ferritin antibody complexesCellular and Molecular Life Sciences, 1975
- A Revised Tannin-Osmium Method for Non-Coated Scanning Electron Microscope SpecimensArchivum histologicum japonicum, 1974