Characterization of Phosphoglycerides by Chemical and Enzymatic Hydrolysis on Thin Layer Plates In Situ
- 1 June 1987
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 10 (8-9) , 1687-1705
- https://doi.org/10.1080/01483918708066794
Abstract
The use of chemical and enzymatic reactions in situ on thin layer chromatograms for characterization of phosphoglycerides is described. Acid and alkaline hydrolysis under specific conditions can differentiate acyl, alkyl and alkenyl moieties. Phospholipases A2, C and D were shown to give complete and specific hydrolysis of phospholipids directly on the chromatograms.Keywords
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