Immunohistochemical demonstration of carbonic anhydrase
- 1 January 1978
- journal article
- research article
- Published by Springer Nature in Histochemistry and Cell Biology
- Vol. 58 (3) , 183-192
- https://doi.org/10.1007/bf00495718
Abstract
Rabbits were immunized using human erythroxyte carbonic anhydrase B (HCA B) purified. by the modified methods of Armstrong et al. (1966) and Bernstein and Schraer (1972). The globulin fraction was isolated by ammonium sulphate precipitation. The anti-HCA B globulin was specific, when judged using the double diffusion technique of Ouchterlony and immunoelectrophoresis. No cross reaction with human erythrocyte carbonic anhydrase C was found, but cross reactions with erythrocyte carbonic anhydrase from rat, mouse and guinea pig were observed. Fluorescein isothiocyanate conjugated goat anti-rabbit globulin was used for the localization of HCA B in tissue sections and erythrocytes on slides.This publication has 36 references indexed in Scilit:
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