Quantitative assay of theN-methylated metabolites of tryptamine and serotonin by gas chromatography mass spectrometry as applied to the determination of lung indoleethylamineN-methyltransferase activity

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Abstract
A specific and sensitive method is described for the identification and quantification of the N‐mono‐ and dimethylated derivatives of tryptamine and serotonin by gas chromatography mass spectrometry. Deuterated analogues of the amines have been prepared for use as internal standards. The technique has been applied to the determination of indoleethylamine N‐methyltransferase activity in rabbit and human lung. No interference from the β‐carboline formation or other side reactions between the substrates and the methyl donor was observed.