STUDIES ONIN VITROPERFUSION OF BEEF AND DOG THYROID GLANDS USING RADIOIODINE1

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Abstract
THE maintenance of the thyroid gland in vitro, by means of vascular perfusion with a medium containing radioactive iodide, might provide a means of evaluating the direct effect of various substances on thyroid function. Roche and associates (1951) perfused beef thyroids with beef serum containing radioactive iodide in order to prepare radioactive thyroglobulin. Under aseptic conditions the beef thyroid collected I131 for 24 hours. After 5 hours of perfusion, radiothyroxine could be demonstrated in the thyroid. The purpose of this study was to delimit the general applicability of in vitro perfusion of the thyroid to problems in thyroid physiology. The thyroid glands of the beef and dog were perfused under nonsterile conditions, and a survey was made of the in vitro effect of thyroid-stimulating and thyroid-inhibiting agents. Radioactive iodine was used to measure various aspects of thyroidal function.