Effect of gamma radiation at high- and low-dose rate on a novel in vivo mutation assay in mouse intestine

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Abstract
We have previously proposed that an in vivo mutagencity assay could be based on the detection of mutations affecting the Dlb-1 locus in the small intestine of C57BL/6J x SWR F1 mice. F1 mice are heterozygous Dlb−1b/Dlb−1a and have only a single allele (Dlb−1b) which specifies expression of the binding site for the lectin Dolichos biflorus agglutinin (DBA) in intestinal epithelia. In whole-mount preparations of small intestine stained with a DBA-peroxidase conjugate, mutated stem cells and their progeny can be recognized as ribbons of unstained cells against a stained background. These DBA-negative ribbons can be quantified. The present investigation characterizes the responses of F1 mice to high-and low-dose rate gamma radiation (1.8 and 0.01 Gy/min respectively) and shows that the induction of ribbons is dose dependent in both cases. Dose sparing is evident at the lower dose rate: 20 ribbons/104 villi can be induced by 4 Gy at high-dose rate and by 7.1 Gy at low-dose rate, a dose sparing of 1.76. Age-matched untreated mice had a background level of 3.7 ribbons/104 villi. As expected, no ribbons were observed in homozygous (Dlb−1b/Dlb−1b) C67BL/6J mice, in which each stem cell has two alleles specifying lectin binding. The results further validate this system as a sensitive in vivo mutagenicity assay and suggests that the target cells are capable of repairing radiation damage.