The Postnatal Ontogeny of Rat Uterine Ornithine Decarboxylase: Acquisition of a Second Peak of Estrogen-Induced Enzyme Activity

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Abstract
Uterine ornithine decarboxylase (ODC) activity is reported to increase after estrogen administration to fetal, neonatal, immature and adult rats, suggesting that it may be a useful marker in studies of the development of estrogen responsiveness. Standard conditions were validated for enzyme assay of uterine cytosols form 5-day-old rats, and it was demonstrated that full activity was retained after freezing cytosol in liquid N2. Maximal activity, obtained 6 h after the injection of 10 .mu.g estradiol (E2) to 5-day-old rats, was also elicited by the same dose of mestranol, ethynylestradiol, diethylstilbestrol or moxestrol. Progesterone, testosterone and low doses of the antiestrogens clomiphene and tamoxifen failed to alter background ODC levels, while high antiestrogen doses induced small increases in enzyme activity. The glucocorticoid prednisolone lowered ODC activity. Dose-response curves established that E2 was more effective in increasing adult ODC levels (ED50 = 0.2 .mu.g/kg E2) than neonatal ODC levels (ED50 = 2 .mu.g/kg E2). Time-course measurements were conducted over 24 h in control and E2-injected animals on postnatal days 5, 10, 14, 20 and 28 and in 60-day-old ovariectomized adults. While an age-dependent decrease in control and 6 h E2-induced ODC levels was observed, there was an unexpected progressive development by day 28 of a 2nd peak of E2-induced ODC at 15-18 h. The 6 h neonatal and 6 and 15-18 h adult ODC peaks had apparent Km values for ornithine near 0.2 mM. The potential origin of the 2nd peak and its relationship to other uterine events are discussed.