Ploidy Effects in Isogenic Populations of Alfalfa
Open Access
- 1 December 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 70 (6) , 1704-1709
- https://doi.org/10.1104/pp.70.6.1704
Abstract
The influence of polyploidization on ribulose-1,5-bisphosphate carboxylase (RuBPCase), buffer-soluble protein (BSP), chlorophyll (Chl), and DNA was examined in fully expanded leaves of isogenic diploid-tetraploid (DDC 2X-4X) and tetraploid-octoploid (IC 4X-8X) sets of alfalfa (Medicago sativa L.). The concentration of RuBPCase in leaf extracts was determined by rocket immunoelectrophoresis. Activities of RuBPCase, expressed per milligram protein or per milligram Chl, and leaf tissue concentrations of RuBPCase, BSP, Chl, and DNA were similar between ploidy levels of the DDC 2X-4X set. Tetraploids and octoploids were similar in RuBPCase activities, expressed per milligram protein or per milligram Chl, and in leaf tissue concentrations of RuBPCase and DNA. Octoploids were significantly lower than tetraploids in concentrations of Chl and BSP. When compared on a per leaf basis, tetraploids were 80% higher in BSP and essentially double comparable diploids in fresh weight, RuBPCase, Chl, and DNA. The observation that leaves of the DDC tetraploid population contain twice as much DNA as comparable diploids suggests that leaves of both ploidy levels contain similar numbers of cells. Leaves of the octoploid population were 33% to 80% higher than corresponding tetraploids in BSP, fresh weight, RuBPCase, Chl, and DNA. Ratios of RuBPCase to DNA and Chl to DNA were similar across ploidy levels of both isogenic sets suggesting that cellular content of Chl and RuBPCase increases proportionately with the amount of DNA per cell.This publication has 24 references indexed in Scilit:
- Ploidy Effects in Isogenic Populations of AlfalfaPlant Physiology, 1982
- Ploidy Effects on Anatomy and Gas Exchange of Tall Fescue LeavesPlant Physiology, 1981
- Enzymic and physicochemical characterization of ribulose 1,5-bisphosphate carboxylase/oxygenase from diploid and tetraploid cultivars of perennial ryegrassArchives of Biochemistry and Biophysics, 1981
- Light limitation of photosynthesis and activation of ribulose bisphosphate carboxylase in wheat seedlingsProceedings of the National Academy of Sciences, 1981
- Similarity of Ribulose-1,5-bisphosphate Carboxylases of Isogenic Diploid and Tetraploid Ryegrass (Lolium perenne L.) CultivarsPlant Physiology, 1981
- Photosynthetic and Photorespiratory Carbon Metabolism in Mesophyll Protoplasts and Chloroplasts Isolated from Isogenic Diploid and Tetraploid Cultivars of Ryegrass (Lolium perenne L.)Plant Physiology, 1980
- Euploidy in Ricinus. I. Euploidy and gene dosage effects on cellular proteinsBiochemical Genetics, 1980
- d-Ribulose-1,5-bisphosphate carboxylase-oxygenaseAnalytical Biochemistry, 1977
- A Rapid and Sensitive Method for the Quantitation of Microgram Quantities of Protein Utilizing the Principle of Protein-Dye BindingAnalytical Biochemistry, 1976
- 23. Immunization, Isolation of Immunoglobulins, Estimation of Antibody TitreScandinavian Journal of Immunology, 1973