Glycolic Acid Oxidase Activity in Cell-free Preparations of Blue-Green Algae
- 1 June 1970
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 45 (6) , 735-737
- https://doi.org/10.1104/pp.45.6.735
Abstract
Glycolic acid oxidase activity was detected in cell-free preparations of Anabaena flos-aquae and Oscillatoria sp. by the reduction of 2,6-dichlorophenolindophenol and by the formation of glyoxylate. Enzyme activity was localized in the 20,000 times gravity supernatant fraction, and optimal activity was obtained at pH 8.0. Activity was lost on storing the preparation at 4 C and could not be restored by addition of flavin mononucleotide.Keywords
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