Shift from fetal‐type to Alzheimer‐type phosphorylated Tau proteins in SKNSH‐SY 5Y cells treated with okadaic acid
- 3 January 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 357 (2) , 197-201
- https://doi.org/10.1016/0014-5793(94)01361-4
Abstract
Tau proteins are abnormally phosphorylated in Alzheimer's disease. Pathological Tau proteins named PHF-Tau 55, PHF-Tau 64, and PHF-Tau 69, are the main constituents of the paired helical filaments (PHF). When treating SKNSH-SY 5Y cells with okadaic acid (OA), Tau 55 protein was clearly induced whereas Tau 64 protein was only faintly induced. Here, we show that the absence of Tau 69 could be explained by the fact that adult isoforms containing N-terminal inserts are not detected. Phosphorylation is similar for untreated cellular Tau proteins and fetal Tau proteins, while OA cell treatment transformed fetal-type into Alzheimer-type phosphorylated proteinsKeywords
This publication has 26 references indexed in Scilit:
- Abnormal Alzheimer‐like phosphorylation of tau‐protein by cyclin‐dependent kinases cdk2 and cdk5Published by Wiley ,1993
- Abnormal tau phosphorylation at Ser396 in alzheimer's disease recapitulates development and contributes to reduced microtubule bindingNeuron, 1993
- Characterization of Two Distinct Monoclonal Antibodies to Paired Helical Filaments: Further Evidence for Fetal‐Type Phosphorylation of the τ in Paired Helical FilamentsJournal of Neurochemistry, 1993
- Okadaic acid induces hyperphosphorylated forms of tau protein in human brain slicesAnnals of Neurology, 1993
- Glycogen synthase kinase‐3 and the Alzheimer‐like state of microtubule‐associated protein tauFEBS Letters, 1992
- Glycogen synthase kinase-3 induces Alzheimer's disease-like phosphorylation of tau: Generation of paired helical filament epitopes and neuronal localisation of the kinaseNeuroscience Letters, 1992
- Implication of brain cdc2 and MAP2 kinases in the phosphorylation of tau protein in Alzheimer's diseaseFEBS Letters, 1992
- Fetal‐Type Phosphorylation of the τ in Paired Helical FilamentsJournal of Neurochemistry, 1992
- Alzheimer disease proteins (A68) share epitopes with tau but show distinct biochemical propertiesJournal of Neuroscience Research, 1990
- Abnormal tau species are produced during Alzheimer's disease neurodegenerating processFEBS Letters, 1989