Mouse-human heterokaryon analysis with a 33258 Hoechst-Giemsa technique.
Open Access
- 1 September 1975
- journal article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 66 (3) , 676-680
- https://doi.org/10.1083/jcb.66.3.676
Abstract
The bibenzimidazol derivative 33258 Hoechst can be used to distinguish microfluorometrically between mouse and human nuclei in heterokaryons. This affords a quick and accurate alternative to autoradiography in the analysis of such heterokaryons. The 33258 Hoechst fluorescence patterns can be converted after irradiation to a Giemsa rendition of the differential staining.Keywords
This publication has 20 references indexed in Scilit:
- New Giemsa method for the differential staining of sister chromatidsNature, 1974
- Localization of Sister Chromatid Exchanges in Human ChromosomesScience, 1974
- Fluorometric properties of the bibenzimidazole derivative hoechst 33258, a fluorescent probe specific for AT concentration in chromosomal DNAChromosoma, 1974
- Microfluorometric Detection of Deoxyribonucleic Acid Replication in Human Metaphase ChromosomesProceedings of the National Academy of Sciences, 1973
- Decondensation of constitutive heterochromatin in L cell chromosomes by a benzimidazole compound (“33258 Hoechst”)Experimental Cell Research, 1973
- Staining of constitutive heterochromatin in mammalian chromosomes with a new fluorochromeExperimental Cell Research, 1972
- Arrangement of centromeres in mouse cellsChromosoma, 1971
- Chromosomal Localization of Mouse Satellite DNAScience, 1970
- The isolation of complementary strands from a mouse DNA fraction.Proceedings of the National Academy of Sciences, 1967
- CLONAL GROWTH OF MAMMALIAN CELLS IN VITROThe Journal of Experimental Medicine, 1956