Chemical transformation of human revertant cells induced by murine sarcoma virus

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Abstract
A human revertant cell line, derived from non‐producer human osteosarcoma cells (NP/KHOS) induced by Kirsten murine sarcoma virus, was treated in vitro with various levels of polycyclic aromatic hydrocarbons (3‐methylcholanthrene, 7,12‐dimethylbenz(a)anthracene, and benzo(a)pyrene [BP] or dimethyl sulfoxide (control). Cells treated only with 3‐methylcholanthrene and 7,12‐dimethyl‐benz(a)anthracene underwent morphological alteration in vitro, and produced tumors when injected into NIH nude mice. These human revertant cells may be a useful in vitro tool in screening for the potentital chemical carcinogens in human cell systems.