Renal Localization of 99m Tc-Stannous Glucoheptonate and 99m Tc-Stannous Dimercaptosuccinate in the Rat by Frozen Section Autoradiography: The Efficiency and Resolution of Technetium-99m

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Abstract
Renal localization of 2 99mTc-labeled radiopharmaceuticals, 99mTc-dimercaptosuccinate and 99mTc-glucoheptonate, 1 h after injection into rats, was studied using frozen section microautoradiography and macroautoradiography (X-ray film autoradiography). The distribution of both compounds was identical within each method, but the patterns of localization between the methods were discordant. Microautoradiographs showed a well-defined and regionally specific pattern of localization in the inner renal cortex, whereas macroautoradiographs demonstrated localization throughout the cortex. In the microautoradiographs the label was found principally over the cytoplasm of proximal tubule cells. Resolution and efficiency data were determined using autoradiographs of radioactive discs. The value for resolution was 2.3 .mu.m and that for efficiency 11%. The radiation dosage experienced by microscopic structures after labeling with 99mTc-radiopharmaceuticals can be calculated from autoradiographs if the efficiency is known.

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