Accumulation of acyl-enzyme in dd-peptidase-catalysed reactions with analogues of peptide substrates
- 1 December 1991
- journal article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 280 (2) , 499-506
- https://doi.org/10.1042/bj2800499
Abstract
Thioester substrates can be used to study the hydrolysis and transfer reactions catalysed by beta-lactamases and DD-peptidases. With the latter enzymes, accumulation of the acyl-enzyme can be detected directly. The efficiency of various amines as acceptor substrates was in excellent agreement with previous results obtained with peptide substrates of the DD-peptidases. The results indicated the presence of a specific binding site for the acceptor substrates. Although most of the results agreed well with a simple partition model, more elaborate hypotheses will be needed to account for all the data presented.Keywords
This publication has 17 references indexed in Scilit:
- The diversity of the catalytic properties of class A β-lactamasesBiochemical Journal, 1990
- beta-Lactamase-catalyzed hydrolysis of acyclic depsipeptides and acyl transfer to specific amino acid acceptors.Proceedings of the National Academy of Sciences, 1984
- Mode of interaction between β-lactam antibiotics and the exocellular DD-carboxypeptidase–transpeptidase from Streptomyces R39.Biochemical Journal, 1976
- Kinetics of Interaction between the Exocellular DD‐Carboxypeptidase‐Transpeptidase from Streptomyces R61 and β‐Lactam AntibioticsEuropean Journal of Biochemistry, 1975
- Molecular weight, amino acid composition and physicochemical properties of the exocellular dd-carboxypeptidase–transpeptidase of Streptomyces R39Biochemical Journal, 1974
- Fluorescence and circular-dichroism studies on the Streptomyces R61 dd-carboxypeptidase–transpeptidase. Penicillin binding by the enzymeBiochemical Journal, 1973
- Kinetics of concomitant transfer and hydrolysis reactions catalysed by the exocellular dd-carboxypeptidase–transpeptidase of Streptomyces R61Biochemical Journal, 1973
- Enzymic mechanisms involving concomitant transfer and hydrolysis reactionsBiochemical Journal, 1973
- Streptomyces dd-carboxypeptidase as transpeptidases. The specificity for amino compounds acting as carboxyl acceptorsBiochemical Journal, 1973
- Penicillin-sensitive DD-carboxypeptidase from Streptomyces strain R 61Biochemistry, 1971