Induction of protein kinase C activation and Ca2+ mobilization by fibroblast growth factor in Swiss 3T3 cells
Open Access
- 28 October 1985
- journal article
- Published by Wiley in FEBS Letters
- Vol. 191 (2) , 205-210
- https://doi.org/10.1016/0014-5793(85)80009-0
Abstract
Addition of fibroblast growth factor (FGF) to quiescent cultures of Swiss 3T3 cells rapidly induced diacylglycerol formation, protein kinase C activation and Ca2+ mobilization. Protein kinase C‐activating agents such as 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) and 1‐oleoyl‐2‐acetylglycerol (OAG) mimicked the action of FGF and stimulated DNA synthesis in the presence of insulin. Prolonged treatment of the cells with phorbol‐12,13‐dibutyrate (PDBu) led to the down‐regulation and complete disappearance of protein kinase C. In these cells, TPA and OAG did not induce DNA synthesis any more. FGF still elicited Ca2+ mobilization and DNA synthesis, but the magnitude of DNA synthesis was reduced to almost half as compared with that in the control cells. These results clearly indicate that both diacylglycerol and Ca2+ may serve as second messengers for FGF and suggest that these messengers may be involved in the mitogenic action of this growth factor.Keywords
This publication has 24 references indexed in Scilit:
- Involvement of Ca2+ in platelet‐derived growth factor‐induced expression of c‐myc oncogene in Swiss 3T3 fibroblastsFEBS Letters, 1985
- Isolation of pituitary fibroblast growth factor by fast protein liquid chromatography (FPLC): Partial chemical and biological characterizationJournal of Cellular Physiology, 1985
- Inositol trisphosphate, a novel second messenger in cellular signal transductionNature, 1984
- Disappearance of Ca2+-sensitive, phospholipid-dependent protein kinase activity in phorbol ester-treated 3T3 cellsBiochemical and Biophysical Research Communications, 1984
- Phorbol esters and vasopressin stimulate DNA synthesis by a common mechanismNature, 1980
- Unsaturated diacylglycerol as a possible messenger for the activation of calcium-activated, phospholipid-dependent protein kinase systemBiochemical and Biophysical Research Communications, 1979
- Early effects of serum on phospholipid metabolism in untransformed and oncogenic virus-transformed cultured fibroblastsBiochemical and Biophysical Research Communications, 1976
- Fibroblast growth factor can substitute for platelet factor to sustain the growth of BALB/3T3 cells in the presence of plasmaBiochemical and Biophysical Research Communications, 1975
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959
- A RAPID METHOD OF TOTAL LIPID EXTRACTION AND PURIFICATIONCanadian Journal of Biochemistry and Physiology, 1959