A Definitive Cloning Technique for Human Fibroblast Cultures.
- 1 October 1966
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 123 (1) , 138-140
- https://doi.org/10.3181/00379727-123-31423
Abstract
Summary Established human fibroblast cultures were cloned by isolating single cells on 3 mm square coverslips and growing them in medium containing pre-tested fetal calf serum. Coverslips with single cells were readily obtained by dilute plating combined with mechanical removal of surplus cells. The mean cloning efficiency was 19.1%. Sub-clones were obtained with a mean frequency of 23%.This publication has 6 references indexed in Scilit:
- The limited in vitro lifetime of human diploid cell strainsPublished by Elsevier ,2004
- A Simplified Method for the Cloning of Heteroploid and Diploid Mammalian Cells.Experimental Biology and Medicine, 1964
- Use of Tris(Hydroxymethyl)Aminomethane Buffers in Cultures of Diploid Human Fibroblasts.Experimental Biology and Medicine, 1964
- Cloning of mammalian cells by a simplified capillary techniqueExperimental Cell Research, 1961
- Rapid Proliferation of Sublines of NCTC Clone 929 (Strain L) Mouse Cells in a Simple Chemically Defined Medium (MB 752/1)2JNCI Journal of the National Cancer Institute, 1959
- Method for Isolating Single Cells and Preparation of Clones from Human Bone Marrow Cultures.Experimental Biology and Medicine, 1958