A RADIOIMMUNOASSAY OF OESTRONE IN PLASMA. PLASMA LEVELS OF OESTRONE AND OESTRADIOL IN OOPHORECTOMIZED RHESUS MONKEYS DURING TREATMENT WITH SUBCUTANEOUS IMPLANTS CONTAINING OESTRONE

Publisher Website
Google Scholar
Abstract
A highly specific antiserum to estrone was used to develop a sensitive radioimmunoassay [RIA]. Plasma was generally analyzed without prior separation of the estrogens. A small amount of plasma (0.1-0.2 ml) was extracted once with diethyl ether. The above and previously described RIA were used to estimate plasma levels of estrogens in oophorectomized monkeys treated with s.c. estrone implants. Three monkeys received a rod containing 40 mg of estrone. The rods were kept in place for about 1 yr. The release rates from the rods were calculated. The estrone plasma levels were initially high (370-500 pg/ml). In about 3 mo. they had fallen to a level of 50-100 pg/ml which was maintained till the removal of the rods. A rise in the estradiol plasma levels was also observed. Rods are suitable for administration of estrone.

This publication has 3 references indexed in Scilit: