Autogenous control of Escherichia coli ribosomal protein L10 synthesis in vitro.
- 1 May 1980
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 77 (5) , 2592-2595
- https://doi.org/10.1073/pnas.77.5.2592
Abstract
The DNA-dependent in vitro synthesis of Escherichia coli ribosomal protein L10 was inhibited when L10 was added to the protein-synthesizing incubations. Addition of L10 had little or no effect on the synthesis of ribosomal protein L12, elongation factor Tu (tufB), or the beta and beta' subunits of RNA polymerase. In addition, ribosomal protein L12 did not inhibit its own synthesis or the synthesis of L10. Experiments using a mRNA-directed system showed that the inhibition of the synthesis of L10 by itself is at the level of translation of protein synthesis. The mechanism of inhibition does not appear to be due to increased degradation of L10 mRNA.Keywords
This publication has 15 references indexed in Scilit:
- In vitro regulation of DNA-dependent synthesis of Escherichia coli ribosomal protein L12.Proceedings of the National Academy of Sciences, 1979
- Autogenous translational repression of bacteriophage T4 gene 32 expression in vitroJournal of Molecular Biology, 1978
- Autogenous regulation of RNA polymerase beta subunit synthesis in vitroJournal of Biological Chemistry, 1978
- mRNA-dependent in vitro synthesis of ribosomal proteins L12 and L10 and elongation factor Tu.Proceedings of the National Academy of Sciences, 1977
- GENETICS OF BACTERIAL RIBOSOMESAnnual Review of Genetics, 1977
- Lambda repressor turns off transcription of its own gene.Proceedings of the National Academy of Sciences, 1975
- The stoichiometry of the ribosomal proteins of Escherichia coliMolecular Genetics and Genomics, 1975
- Copies of proteins L7 and L12 and heterogeneity of the large subunit of Escherichia coli ribosomeJournal of Molecular Biology, 1975
- Purification and properties of a soluble factor required for the deoxyribonucleic acid-directed in vitro synthesis of beta-galactosidase.Journal of Biological Chemistry, 1975
- Further Studies on the Role of 50 S Ribosomal Proteins in Protein SynthesisJournal of Biological Chemistry, 1973