Inhibition of Human Renal Cancer by Methotrexate Linked to a Monoclonal Antibody
- 1 February 1989
- journal article
- research article
- Published by Wolters Kluwer Health in Journal of Urology
- Vol. 141 (2) , 428-431
- https://doi.org/10.1016/s0022-5347(17)40787-7
Abstract
The monoclonal antibody DAL K29 against a human renal cell carcinoma associated cell surface antigen was covalently linked to the antifolate methotrexate with full retention of antibody reactivity and partial retention of drug activity. In a colony inhibition assay, antibody-conjugated methotrexate was 400% more potent in inhibiting the growth of the human kidney cancer line Caki-1 than equimolar amounts of the free drug. Comparable amounts of the antifolate linked to normal mouse IgG did not inhibit the growth of Caki-1 cells. Furthermore, the methotrexate-DAL K29 conjugate had no effect on the two nontarget human cell lines tested, melanoma M21 and B cell leukemia D10-1 cells, even when the conjugate contained amounts of methotrexate equivalent to the 50% inhibitory concentration of the conjugated drug for Caki-1 cells.This publication has 11 references indexed in Scilit:
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