Assay for Antibody Mediated Cytotoxicity of Mouse Spermatozoa by 86Rubidium Release
- 1 March 1979
- journal article
- research article
- Published by Wiley in Tissue Antigens
- Vol. 13 (3) , 177-185
- https://doi.org/10.1111/j.1399-0039.1979.tb00781.x
Abstract
The analysis of the antigenic structure of murine spermatozoa has been hampered by the lack of a convenient objective serological assay. Spermatozoa, in common with other cells, are capable of concentrating 86Rb. Antibody mediated complement dependent cytotoxicity of murine spermatozoa was quantitated by measuring release of 86Rb from pre-labeled spermatozoa. This assay was used to study the murine T/t locus.This publication has 17 references indexed in Scilit:
- Comparative evaluation of the isotopes 86Rb and 51Cr in a humoral cytotoxicity systemJournal of Immunological Methods, 1974
- Serological identification of sperm antigens specified by lethalt-alleles in the mouseImmunogenetics, 1974
- Transmission ratio distortion at the T-locus: Serological identification of two sperm populations int-heterozygotesImmunogenetics, 1974
- H-2 ANTIGENS ON MOUSE SPERMATOZOATransplantation, 1972
- Serological Demonstration of H–Y (Male) Antigen on Mouse SpermNature, 1971
- The comparative transport of K+ and Rb+ in normal and malignant rat tissuesin vivo and in liver slices, diaphragm, and tumor slicesin vitroThe Journal of Membrane Biology, 1970
- IMPROVED COMPLEMENTATION IN THE CYTOTOXIC TESTTransplantation, 1970
- Detection of H-2 Antigens on Mouse Spermatozoa by the Cytotoxicity TestNature, 1970
- H-2d Antigens on Mouse SpermatozoaNature, 1969
- Antigenic Differences Between the Sperm of Different Inbred Strains of MiceScience, 1944