Ectogalactosyltransferase studies in fibroblasts and concanavalin A-stimulated lymphocytes.
Open Access
- 1 March 1976
- journal article
- research article
- Published by Rockefeller University Press in The Journal of cell biology
- Vol. 68 (3) , 799-802
- https://doi.org/10.1083/jcb.68.3.799
Abstract
In this communication, we have demonstrated that hydrolysis of the nucleotide sugar can cause errors in the detection of an ectoglycosyltransferase. Spleen cell suspensions can incorporate radioactivity when incubated with labeled UDP-galactose, but all the activity is due to decomposition of the nucleotide sugar and uptake of the free sugar. The fibroblast cell lines can incroporate carbohydrate directly from UDP-galactose. Several criteria are presented with can be used to demonstrate that a nucleotide sugar is the direct carbohydrate donor.This publication has 12 references indexed in Scilit:
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