Determination of Thiamphenicol in Serum and Cerebrospinal Fluid with High-Pressure Liquid Chromatography
- 1 January 1981
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 4 (1) , 145-154
- https://doi.org/10.1080/01483918108064804
Abstract
A procedure for quantitation of thiamphenicol in serum and CSF was developed using high-pressure liquid chromatography. The drug was extracted from biological samples with methanol and separated by reverse-phase high-pressure liquid chromatography. Detection and subsequent quantitation were performed at 254 nm by on-line UV spectrophotometry. After the i.m. administration of a single dose of 1 g of thiamphenicol to a patient, a poor transmission of the drug across the hematoencephalic barrier was demonstrated by this assay.This publication has 10 references indexed in Scilit:
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