The Demand for Iodine and Bromine of three Marine Brown Algae grown in Bacteria‐free Cultures

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Abstract
Three marine brown algae have been cultivated with different additions of iodine and bromine in bacteria‐free cultures. Ectocarpus jasciculatus appeared to have an absolute demand for iodine and was inhibited by a concentration of 64 μmol of KJ per 1. Lithosiphon pusillus had the best growth in the highest concentration tested (64 μmol/1) but there was always some growth in the series without iodine. Additions could be made either as inorganic iodine or as organically bound iodine. Additions of KJ to a culture medium consisting of vitamin‐free Asp 6 F with B12 (1 μg/1) and kinetin (20 μmol/1) remarkably increased the growth of the zoospores of Pylaiella litoralis. Lithosiphon pusilius proved to be indifferent to bromide additions in media containing KJ. In media lacking KJ addition of 1 μmol of KBr per 1 is stimulating but higher concentrations of KBr are inhibiting. The inhibiting effect is overcome by iodide addition.