Circularized Chromosome with a Large Palindromic Structure inStreptomyces griseusMutants
Open Access
- 1 June 2004
- journal article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 186 (11) , 3313-3320
- https://doi.org/10.1128/jb.186.11.3313-3320.2004
Abstract
Streptomyceslinear chromosomes display various types of rearrangements after telomere deletion, including circularization, arm replacement, and amplification. We analyzed the new chromosomal deletion mutantsStreptomyces griseus301-22-L and 301-22-M. In these mutants, chromosomal arm replacement resulted in long terminal inverted repeats (TIRs) at both ends; different sizes were deleted again and recombined inside the TIRs, resulting in a circular chromosome with an extremely large palindrome. Short palindromic sequences were found in parent strain 2247, and these sequences might have played a role in the formation of this unique structure. Dynamic structural changes ofStreptomyceslinear chromosomes shown by this and previous studies revealed extraordinary strategies of members of this genus to keep a functional chromosome, even if it is linear or circular.Keywords
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